Dengue Virus Net is the web resource for anyone interested in dengue. The objectives of Dengue Virus Net are to be the public and professional information resource for dengue and to serve as a network in the exchange of information and news related to dengue.
Dengue is a mosquito-borne infection that causes a severe flu-like illness (dengue fever), and sometimes a potentially lethal complication called dengue haemorrhagic fever. Global incidence of dengue has grown dramatically in recent decades. About two fifths of the world's population are now at risk. Dengue is found in tropical and sub-tropical climates worldwide, mostly in urban and semi-urban areas. Dengue is transmitted to humans by the Aedes aegypti or more rarely the Aedes albopictus mosquito, which feed during the day. Dengue haemorrhagic fever is a leading cause of serious illness and death among children in some Asian countries. There is currently no specific treatment for dengue.
- Chinese Health Authorities Launch Campaign to Fight Dengue Fever Outbreak - RIA Novosti
Tue, 30 Sep 2014 17:48:
- Dengue cases in Northern Mindanao continue to rise - Sun.Star
Tue, 30 Sep 2014 16:37:
- Makati dengue cases drop - Manila Standard Today
Tue, 30 Sep 2014 16:21:
- China campaigns against dengue fever outbreak - Shanghai Daily (subscription)
Tue, 30 Sep 2014 12:46:
- China battles worst dengue outbreak in 20 years - IBNLive
Tue, 30 Sep 2014 13:15:
- South China Province Sees 1152 New Dengue Fever Cases - MENAFN.COM
Tue, 30 Sep 2014 13:10:
- Papaya leaf dengue treatment - The Sun Daily
Tue, 30 Sep 2014 13:06:
- Bhopal: Centre's expert dissatisfied with efforts against dengue - Hindustan Times
Tue, 30 Sep 2014 12:07:
- CDC: China takes action against dengue fever - CCTV
Tue, 30 Sep 2014 02:26:
- Venezuela Invests US$66 million to Battle Chikungunya and Dengue - teleSUR English
Tue, 30 Sep 2014 04:00:
- Association of HLA Class-I and Inhibitory KIR Genotypes in Gabonese Patients Infected by Chikungunya or Dengue Type-2 Viruses.
Petitdemange C, Wauquier N, Jacquet JM, et al. Association of HLA Class-I and Inhibitory KIR Genotypes in Gabonese Patients Infected by Chikungunya or Dengue Type-2 Viruses. [JOURNAL ARTICLE]PLoS One 2014; 9(9):e108798.Natural killer (NK) cells provide defense in the early stages of the immune response against viral infections. Killer cell immunoglobulin-like receptors (KIR) expressed on the surface of NK cells play an important role in regulating NK cell response through recognition of human leukocyte antigen (HLA) class I molecules on target cells. Previous studies have shown that specific KIR/ligand combinations are associated with the outcome of several viral infectious diseases.We investigated the impact of inhibitory and activating KIR and their HLA-class I ligand genotype on the susceptibility to Chikungunya virus (CHIKV) and Dengue virus (DENV2) infections. From April to July 2010 in Gabon, a large outbreak of CHIKV and DENV2 concomitantly occurred in two provinces of Gabon (Ogooué-Lolo and Haut-Ogooué). We performed the genotypic analysis of KIR in the combination with their cognate HLA-class I ligands in 73 CHIKV and 55 DENV2 adult cases, compared with 54 healthy individuals.We found in CHIV-infected patients that KIR2DL1 and KIR2DS5 are significantly increased and decreased respectively, as compared to DENV2+ patients and healthy donors. The combination of KIR2DL1 and its cognate HLA-C2 ligand was significantly associated with the susceptibility to CHIKV infection. In contrast, no other inhibitory KIR-HLA pairs showed an association with the two mosquito-borne arboviruses.These observations are strongly suggestive that the NK cell repertoire shaped by the KIR2DL1:HLA-C2 interaction facilitate specific infection by CHIKV.
- Human Vaccines & Immunotherapeutics: News.
Riedmann EM Human Vaccines & Immunotherapeutics: News. [JOURNAL ARTICLE]Hum Vaccin Immunother 2014 Jun 1; 10(6):1427-1430.Sanofi Pasteur shows efficacy of its dengue vaccine in phase 3 New glioblastoma vaccine: Safe and immunogenic in phase 1 FDA approves sublingual hay fever immunotherapeutic Combining the cancer vaccine DPX-Survivac with immune modulators Two Meningococcal B vaccines receive FDA "Breakthrough Therapy" designation MERS vaccine is technically feasible, but is it commerically feasible? Stemline's synthetic multi-peptide cancer vaccine enters Phase 2 Evolution of whooping cough bacterium may reduce vaccine effectiveness.
- Opsoclonus-myoclonus-ataxia syndrome associated with dengue virus infection.
Tan AH, Linn K, Ramli NM, et al. Opsoclonus-myoclonus-ataxia syndrome associated with dengue virus infection. [LETTER]Parkinsonism Relat Disord 2014 Sep 16.
- Identification of a novel inhibitor of dengue virus protease through use of a virtual screening drug discovery web portal.
Viswanathan U, Tomlinson SM, Fonner JM, et al. Identification of a novel inhibitor of dengue virus protease through use of a virtual screening drug discovery web portal. [JOURNAL ARTICLE]J Chem Inf Model 2014 Sep 29.We report the discovery of a novel small molecule inhibitor of the dengue virus (DENV) protease (NS2B-NS3pro) using a newly constructed web-based portal (DrugDiscovery@TACC) for structure-based virtual screening. Our drug discovery portal, an extension of virtual screening studies performed using IBM's World Community Grid, facilitated access to supercomputer resources managed by the Texas Advanced Computing Center (TACC) and enabled drug-like commercially available small molecule libraries to be rapidly screened against several high resolution DENV NS2B-NS3pro crystallographic structures. Detailed analysis of virtual screening docking scores and hydrogen bonding interactions between each docked ligand and the NS2B-NS3pro Ser135 side chain were used to select molecules for experimental validation. Compounds were ordered from established chemical companies and compounds with established aqueous solubility were tested for ability to inhibit DENV NS2B-NS3pro cleavage of a model substrate in kinetic studies. As proof-of-concept, we validated a small molecule dihydro-naphthalenone hit as a single digit micromolar mixed noncompetitive inhibitor of the DENV protease. Since the dihydro-naphthalenone was predicted to interact with NS2B-NS3pro residues that are largely conserved between DENV and the related West Nile virus (WNV), we tested this inhibitor against WNV NS2B-NS3pro and observed a similar mixed noncompetitive inhibition mechanism. However, the inhibition constants were ~10-fold larger against the WNV protease relative to the DENV protease. This novel validated lead had no chemical features or pharmacophores associated with adverse toxicity, carcinogenicity, or mutagenicity risks and thus is attractive for additional characterization and optimization.
- Thioaptamers Targeting Dengue Virus Type-2 Envelope Protein Domain III.
Gandham SH, Volk DE, Rao LG, et al. Thioaptamers Targeting Dengue Virus Type-2 Envelope Protein Domain III. [JOURNAL ARTICLE]Biochem Biophys Res Commun 2014 Sep 24.Thioaptamers targeting the dengue-2 virus (DENV-2) envelope protein domain III (EDIII) were developed. EDIII, which contains epitopes for binding neutralizing antibodies, is the putative host-receptor binding domain and is thus an attractive target for development of vaccines, anti-viral therapeutic and diagnostic agents. Thioaptamer DENTA-1 bound to DENV-2 EDIII adjacent to a known neutralizing antibody binding site with a dissociation constant of 154 nM.
- Predicting outcome from dengue.
Yacoub S, Wills B Predicting outcome from dengue. [JOURNAL ARTICLE]BMC Med 2014 Sep 4; 12(1):147.Dengue is emerging as one of the most abundant vector-borne disease globally. Although the majority of infections are asymptomatic or result in only a brief systemic viral illness, a small proportion of patients develop potentially fatal complications. These severe manifestations, including a unique plasma leakage syndrome, a coagulopathy sometimes accompanied by bleeding, and organ impairment, occur relatively late in the disease course, presenting a window of opportunity to identify the group of patients likely to progress to these complications. However, as yet, differentiating this group from the thousands of milder cases seen each day during outbreaks remains challenging, and simple and inexpensive strategies are urgently needed in order to improve case management and to facilitate appropriate use of limited resources. This review will cover the current understanding of the risk factors associated with poor outcome in dengue. We focus particularly on the clinical features of the disease and on conventional investigations that are usually accessible in mid-level healthcare facilities in endemic areas, and then discuss a variety of viral, immunological and vascular biomarkers that have the potential to improve risk prediction. We conclude with a description of several novel methods of assessing vascular function and intravascular volume status non-invasively.
- Preventing dengue and chikungunya fever among international travelers.
Herold Tither P Preventing dengue and chikungunya fever among international travelers. [JOURNAL ARTICLE]J Am Assoc Nurse Pract 2014 Sep 25.To describe the vulnerability of U.S. travelers in tropical and subtropical regions of the world to dengue and chikungunya fever, to provide practical recommendations to avoid these mosquito-borne diseases, and to offer a communication tool as an aid for pretravel health consultations.Medical, epidemiological, and entomological research articles and reviews, and reports from government agencies.Dengue and chikungunya fever have growing public health impact around the world. International travelers return to the United States infected with these diseases. Mosquito bite avoidance is the only way to prevent dengue and chikungunya fever. Informed travelers have many options for simple and practical measures to lessen the risk of mosquito bites. A message map can be used as a communication tool for pretravel counseling on the prevention of dengue and chikungunya fever within the time frame of an office visit.In a pretravel health consultation, a nurse practitioner can promote travelers' health and prevent dengue, chikungunya fever, and other mosquito-borne diseases by counseling on the risk of these diseases and giving practical recommendations for prevention using a message map.
- Integrating the Public in Mosquito Management: Active Education by Community Peers Can Lead to Significant Reduction in Peridomestic Container Mosquito Habitats.
Healy K, Hamilton G, Crepeau T, et al. Integrating the Public in Mosquito Management: Active Education by Community Peers Can Lead to Significant Reduction in Peridomestic Container Mosquito Habitats. [JOURNAL ARTICLE]PLoS One 2014; 9(9):e108504.AbstractPublisher Full TextMosquito species that utilize peridomestic containers for immature development are commonly aggressive human biters, and because they often reach high abundance, create significant nuisance. One of these species, the Asian tiger mosquito Aedes albopictus, is an important vector of emerging infectious diseases, such as dengue, chikungunya, and Zika fevers. Integrated mosquito management (IMM) of Ae. albopictus is particularly difficult because it requires access to private yards in urban and suburban residences. It has become apparent that in the event of a public health concern due to this species, homeowners will have to be active participants in the control process by reducing mosquito habitats in their properties, an activity known as source reduction. However, limited attempts at quantifying the effect of source reduction by homeowners have had mixed results. Of note, many mosquito control programs in the US have some form of education outreach, however the primary approach is often passive focusing on the distribution of education materials as flyers. In 2010, we evaluated the use of active community peer education in a source reduction program, using AmeriCorps volunteers. The volunteers were mobilized over a 4-week period, in two areas with approximately 1,000 residences each in urban Mercer and suburban Monmouth counties in New Jersey, USA. The volunteers were first provided training on peridomestic mosquitoes and on basic approaches to reducing the number of container habitats for mosquito larvae in backyards. Within the two treatment areas the volunteers successfully engaged 758 separate homes. Repeated measures analysis of variance showed a significant reduction in container habitats in the sites where the volunteers actively engaged the community compared to untreated control areas in both counties. Our results suggest that active education using community peer educators can be an effective means of source reduction, and a critical tool in the arsenal against peridomestic mosquitoes.
- Pathogen inactivation technologies for cellular blood components: an update.
Schlenke P Pathogen inactivation technologies for cellular blood components: an update. [Journal Article, Review]Transfus Med Hemother 2014 Jul; 41(4):309-25.Nowadays patients receiving blood components are exposed to much less transfusion-transmitted infectious diseases than three decades before when among others HIV was identified as causative agent for the acquired immunodeficiency syndrome and the transmission by blood or coagulation factors became evident. Since that time the implementation of measures for risk prevention and safety precaution was socially and politically accepted. Currently emerging pathogens like arboviruses and the well-known bacterial contamination of platelet concentrates still remain major concerns of blood safety with important clinical consequences, but very rarely with fatal outcome for the blood recipient. In contrast to the well-established pathogen inactivation strategies for fresh frozen plasma using the solvent-detergent procedure or methylene blue and visible light, the bench-to-bedside translation of novel pathogen inactivation technologies for cell-containing blood components such as platelets and red blood cells are still underway. This review summarizes the pharmacological/toxicological assessment and the inactivation efficacy against viruses, bacteria, and protozoa of each of the currently available pathogen inactivation technologies and highlights the impact of the results obtained from several randomized clinical trials and hemovigilance data. Until now in some European countries pathogen inactivation technologies are in in routine use for single-donor plasma and platelets. The invention and adaption of pathogen inactivation technologies for red blood cell units and whole blood donations suggest the universal applicability of these technologies and foster a paradigm shift in the manufacturing of safe blood.
- Neurologic complications in dengue virus infection: A prospective cohort study.
Sahu R, Verma R, Jain A, et al. Neurologic complications in dengue virus infection: A prospective cohort study. [JOURNAL ARTICLE]Neurology 2014 Sep 24.AbstractPublisher Full TextThis study aimed to evaluate the incidence and clinical spectrum of neurologic complications, predictors of central and peripheral nervous system involvement, and their outcome in patients with dengue virus infection (DENV).To determine the extent of neurologic complications, we used a hospital-based prospective cohort study design, which included laboratory-confirmed cases of dengue and follow-up for 3 months. We also analyzed clinical and laboratory data to assess predictors of neurologic involvement.The study included enrollment of 486 cases. Two were lost to follow-up and excluded. Forty-five patients developed neurologic complications. Of these, 28 patients had CNS and 17 had peripheral nervous system (PNS) involvement, representing an incidence rate for neurologic complications of 9.26%. Significant predictors of CNS involvement were higher mean body temperature (p = 0.012), elevated hematocrit (p = 0.009), low platelet count (p = 0.021), and liver dysfunction (p < 0.001). Predictors of PNS involvement were higher mean body temperature (p = 0.031), rash (p = 0.002), and elevated hematocrit (p < 0.001). The mortality rate was 4.5%. The remainder of the patients recovered.An increasingly wide spectrum and higher incidence of neurologic complications of DENV are reported. Clinical and laboratory parameters such as higher mean body temperature, rash, increases in hematocrit, thrombocytopenia, and liver dysfunction are independent predictors of neurologic complications.