Dengue Virus Net is the web resource for anyone interested in dengue. The objectives of Dengue Virus Net are to be the public and professional information resource for dengue and to serve as a network in the exchange of information and news related to dengue.
Dengue is a mosquito-borne infection that causes a severe flu-like illness (dengue fever), and sometimes a potentially lethal complication called dengue haemorrhagic fever. Global incidence of dengue has grown dramatically in recent decades. About two fifths of the world's population are now at risk. Dengue is found in tropical and sub-tropical climates worldwide, mostly in urban and semi-urban areas. Dengue is transmitted to humans by the Aedes aegypti or more rarely the Aedes albopictus mosquito, which feed during the day. Dengue haemorrhagic fever is a leading cause of serious illness and death among children in some Asian countries. There is currently no specific treatment for dengue.
- A decrease in the dengue epidemic has been witnesses due to measures taken ... - SLBC
Wed, 20 Aug 2014 08:19:
- 1st dengue death reported in Kaohsiung, public urged to take precautions: CDC - China Post
Wed, 20 Aug 2014 07:05:
- Lowe Lintas crafts new Dengue-themed campaign for Godrej HIT - exchange4media.com
Wed, 20 Aug 2014 04:02:
- Dengue fever forces Indonesian to withdraw from worlds - YahooXtra Blogs (blog)
Wed, 20 Aug 2014 06:41:
- Q&A: over two billion people at risk from dengue - The Guardian
Wed, 20 Aug 2014 06:01:
- Badminton - Dengue fever forces Indonesian to withdraw from worlds - Yahoo Eurosport UK
Wed, 20 Aug 2014 05:45:
- Genetically modified mosquitoes to combat dengue - Free Malaysia Today
Wed, 20 Aug 2014 03:04:
- 26 dengue cases reported in Delhi: SDMC - The Hindu
Wed, 20 Aug 2014 02:51:
- Maynilad's Anti-Dengue Bottle wins international award - Philippine Information Agency
Wed, 20 Aug 2014 04:09:
- Delhi Dengue Update: 25 cases reported so far - TheHealthSite
Wed, 20 Aug 2014 03:54:
- Modulation of inflammation and pathology during dengue virus infection by p38 MAPK inhibitor SB203580.
Fu Y, Yip A, Seah PG, et al. Modulation of inflammation and pathology during dengue virus infection by p38 MAPK inhibitor SB203580. [JOURNAL ARTICLE]Antiviral Res 2014 Aug 14.Dengue virus (DENV) infection could lead to dengue fever (DF), dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). The disease outcome is controlled by both viral and host factors. Inflammation mediators from DENV-infected cells could contribute to increased vascular permeability, leading to severe DHF/DSS. Therefore, suppression of inflammation could be a potential therapeutic approach for treatment of dengue patients. In this context, p38 MAPK (mitogen-activated protein kinase) is a key enzyme that modulates the initiation of stress and inflammatory responses. Here we show that SB203580, a p38 MAPK inhibitor, suppressed the over production of DENV-induced pro-inflammatory mediators such as TNF-α, IL-8, and RANTES from human PBMCs, monocytic THP-1, and granulocyte KU812 cell lines. Oral administration of SB203580 in DENV-infected AG129 mice prevented hematocrit rise and lymphopenia, limited the development of inflammation and pathology (including intestine leakage), and significantly improved survival. These results, for the first time, have provided experimental evidence to imply that a short term inhibition of p38 MAPK may be beneficial to reduce disease symptoms in dengue patients.
- IFITM3-containing exosome as a novel mediator for anti-viral response in dengue virus infection.
Zhu X, He Z, Yuan J, et al. IFITM3-containing exosome as a novel mediator for anti-viral response in dengue virus infection. [JOURNAL ARTICLE]Cell Microbiol 2014 Aug 11.Interferon-inducible transmembrane proteins 1, 2 and 3 (IFITM1, IFITM2 and IFITM3) have recently been identified as potent antiviral effectors that function to suppress the entry of a broad range of enveloped viruses and modulate cellular tropism independent of viral receptor expression. However, the antiviral effect and mechanisms of IFITMs in response to viral infections remain incompletely understood and characterized. In this work, we focused our investigation on the function of the extracellular IFITM3 protein. In cell models of DENV-2 infection, we found that IFITM3 contributed to both the baseline and interferon-induced inhibition of DENV entry. Most importantly, our study for the first time demonstrated the presence of IFITM-containing exosome in the extracellular environment, and identified an ability of cellular exosome to intercellularly deliver IFITM3 and thus transmit its antiviral effect from infected to non-infected cells. Thus, our findings provide new insights in the basic mechanisms underlying the actions of IFITM3, which might lead to future development of exosome-mediated anti-viral strategies using IFITM3 as a therapeutic agent. Conceivably, variations in the basal and inducible levels of IFITMs, as well as in intracellular and extracellular levels of IFITMs, might predict the severity of dengue virus infections among individuals or across species.
- Cutaneous manifestations of infection in returning travelers.
Eldridge M, Cohen SH Cutaneous manifestations of infection in returning travelers. [Journal Article]Curr Infect Dis Rep 2014 Oct; 16(10):426.Dermatologic problems are common in returning travelers who seek medical care. Some common illnesses such as cellulitis or abscesses occur in travelers but are not unique to this patient group. Additionally, dermatologic illnesses not due to infection such as drug eruptions or phototoxicity are seen. Skin manifestations occur in many tropical illnesses such as dengue, schistosomiasis, and leishmaniasis. This article reviews the common causes of cutaneous illnesses associated with travel including non-infectious causes, infections that are not limited to travelers, and infections that are almost exclusively seen in travelers.
- Variable effects of the co-administration of a GM-CSF-expressing plasmid on the immune response to flavivirus DNA vaccines in mice.
Chen H, Gao N, Wu J, et al. Variable effects of the co-administration of a GM-CSF-expressing plasmid on the immune response to flavivirus DNA vaccines in mice. [JOURNAL ARTICLE]Immunol Lett 2014 Aug 13.As a cytokine adjuvant, granulocyte-macrophage colony-stimulating factor (GM-CSF) has been demonstrated to play central roles in the enhancement of the immune response and protection elicited by experimental vaccines. However, in our previous work, the co-administration of GM-CSF produced untoward effects on the immune response induced by a Japanese encephalitis virus DNA vaccine candidate. This study aimed to elucidate the adjuvant roles of GM-CSF in several Flaviviridae virus DNA vaccine candidates. Our results showed that the effects of GM-CSF were diverse: coinoculated GM-CSF caused significant suppression to the dengue virus type 1 and type 2 prM-E DNA vaccinations and influenced protective efficiency against virus challenge. In contrast, GM-CSF showed little effect or an enhancement on the immune response elicited by hepatitis C virus C or E1 DNA vaccine candidates. Notably, these effects of GM-CSF were highly durable. Our results suggested that the adjuvant roles of the GM-CSF plasmid were complex and diverse, ranging from enhancement to suppression, depending on the immunogen of Flaviviridae virus DNA vaccine candidates. Therefore, the application of GM-CSF as a vaccine adjuvant or a therapeutic agent should be evaluated carefully.
- Dengue Virus 2 American-Asian Genotype Identified during the 2006/2007 Outbreak in Piauí, Brazil Reveals a Caribbean Route of Introduction and Dissemination of Dengue Virus in Brazil.
Barcelos Figueiredo L, Sakamoto T, Leomil Coelho LF, et al. Dengue Virus 2 American-Asian Genotype Identified during the 2006/2007 Outbreak in Piauí, Brazil Reveals a Caribbean Route of Introduction and Dissemination of Dengue Virus in Brazil. [JOURNAL ARTICLE]PLoS One 2014; 9(8):e104516.Dengue virus (DENV) is the most widespread arthropod-borne virus, and the number and severity of outbreaks has increased worldwide in recent decades. Dengue is caused by DENV-1, DENV- 2, DENV-3 and DENV-4 which are genetically distant. The species has been subdivided into genotypes based on phylogenetic studies. DENV-2, which was isolated from dengue fever patients during an outbreak in Piaui, Brazil in 2006/2007 was analyzed by sequencing the envelope (E) gene. The results indicated a high similarity among the isolated viruses, as well as to other DENV-2 from Brazil, Central America and South America. A phylogenetic and phylogeographic analysis based on DENV-2E gene sequences revealed that these viruses are grouped together with viruses of the American-Asian genotype in two distinct lineages. Our results demonstrate the co-circulation of two American-Asian genotype lineages in northeast Brazil. Moreover, we reveal that DENV-2 lineage 2 was detected in Piauí before it disseminated to other Brazilian states and South American countries, indicating the existence of a new dissemination route that has not been previously described.
- Dengue virus infection induces broadly cross-reactive human IgM antibodies that recognize intact virions in humanized BLT-NSG mice.
Jaiswal S, Smith K, Ramirez A, et al. Dengue virus infection induces broadly cross-reactive human IgM antibodies that recognize intact virions in humanized BLT-NSG mice. [JOURNAL ARTICLE]Exp Biol Med (Maywood) 2014 Aug 14.The development of small animal models that elicit human immune responses to dengue virus (DENV) is important since prior immunity is a major risk factor for developing severe dengue disease. This study evaluated anti-DENV human antibody (hAb) responses generated from immortalized B cells after DENV-2 infection in NOD-scid IL2rγ(null) mice that were co-transplanted with human fetal thymus and liver tissues (BLT-NSG mice). DENV-specific human antibodies predominantly of the IgM isotype were isolated during acute infection and in convalescence. We found that while a few hAbs recognized the envelope protein produced as a soluble recombinant, a number of hAbs only recognized epitopes on intact virions. The majority of the hAbs isolated during acute infection and in immune mice were serotype-cross-reactive and poorly neutralizing. Viral titers in immune BLT-NSG mice were significantly decreased after challenge with a clinical strain of dengue. DENV-specific hAbs generated in BLT-NSG mice share some of the characteristics of Abs isolated in humans with natural infection. Humanized BLT-NSG mice provide an attractive preclinical platform to assess the immunogenicity of candidate dengue vaccines.
- Use and acceptance of long lasting insecticidal net screens for dengue prevention in Acapulco, Guerrero, Mexico.
Jones CH, Benítez-Valladares D, Guillermo-May G, et al. Use and acceptance of long lasting insecticidal net screens for dengue prevention in Acapulco, Guerrero, Mexico. [JOURNAL ARTICLE]BMC Public Health 2014 Aug 14; 14(1):846.Dengue, recognized by the WHO as the most important mosquito-borne viral disease in the world, is a growing problem. Currently, the only effective way of preventing dengue is vector control. Standard methods have shown limited effect, and there have been calls to develop new integrated vector management approaches. One novel tool, protecting houses with long lasting insecticidal screens on doors and windows, is being trialled in a cluster randomised controlled trial by a joint UADY/WHO TDR/IDRC study in various districts of Acapulco, Mexico, with exceptionally high levels of crime and insecurity.This study investigated the community's perspectives of long lasting insecticidal screens on doors and windows in homes and in schools, in order to ascertain their acceptability, to identify challenges to further implementation and opportunities for future improvements.This was a sequential mixed-methods study. The quantitative arm contained a satisfaction survey administered to 288 houses that had received the intervention examining their perspectives of both the intervention and dengue prevention in general. The qualitative arm consisted of Focus Group Discussions (FGDs) with those who had accepted the intervention and key informant interviews with: schoolteachers to discuss the use of the screens in schools, program staff, and community members who had refused the intervention.Overall satisfaction and acceptance of the screens was very high, with only some operational and technical complaints relating to screen fragility and the installation process. However, the wider social context of urban violence and insecurity was a major barrier to screen acceptance. Lack of information dissemination and community collaboration were identified as project weaknesses.The screens are widely accepted by the population, but the project implementation could be improved by reassuring the community of its legitimacy in the context of insecurity. More community engagement and better information sharing structures are needed.The screens could be a major new dengue prevention tool suitable for widespread use, if further research supports their entomological and epidemiological effectiveness and their acceptability in different social and environmental contexts. Further research is needed looking at the impact of insecurity of dengue prevention programmes.
- Antibody response to dengue virus.
Cedillo-Barrón L, Cordero JG, Bustos-Arriaga J, et al. Antibody response to dengue virus. [REVIEW]Microbes Infect 2014 Aug 11.In this review, we discuss the current knowledge of the role of the antibody response against dengue virus and highlight novel insights into targets recognized by the human antibody response. We also discuss how the balance of pathological and protective antibody responses in the host critically influences clinical aspects of the disease.
- [Knowledge about dengue and cardio-cerebrovascular disease in a town in Colombia].
Escobar-Montoya JI [Knowledge about dengue and cardio-cerebrovascular disease in a town in Colombia]. [English Abstract, Journal Article]Rev Salud Publica (Bogota) 2013 Aug; 15(4):676-88.Objective This study aimed at describing a low-income population's knowledge and perception concerning dengue and cardio-cerebrovascular disease in a town in Colombia. Method The study involved qualitative research; data from 16 semi-structured individual and 3 focus groups was collected through questionnaires addressing 3 low-income sectors and then interpreted. Deductive categorisation of data was based on the questions and inductive analysis to establish such population's knowledge for studying their perceptions regarding dengue and cardio-cerebrovascular disease. Results It was evident that the target population knew about the agents, the forms of acquiring such diseases, the risk factors and action to be taken for preventing and controlling both diseases; however, such knowledge did not represent (i.e. had not led to) changes in their daily practice or lifestyles. Conclusions Local health centre actions needs to be supported by theory or models and must go beyond single interventions based essentially on the transmission of information.
- [Epidemiological surveillance, molecular biology and dengue 5].
Ramírez-Jaramillo V, Bedoya-Arias JE, Calvache-Benavides CE, et al. [Epidemiological surveillance, molecular biology and dengue 5]. [Journal Article]Rev Peru Med Exp Salud Publica 2014 Jun; 31(2):394-5.