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Dengue Literature - Latest PubMed Articles

Overview of latest articles and publications on ebola in PubMed. PubMed is a service of the US National Library of Medicine that includes over 18 million citations from MEDLINE and other life science journals.


  • Cross reactivity of commercial anti-dengue immunoassays in patients with acute Zika virus infection.
    Cross reactivity of commercial anti-dengue immunoassays in patients with acute Zika virus infection. [Journal Article]J Med Virol 2017 Feb 23.JMFelix AC, Souza NC, Figueiredo WM, et al. Several countries have local transmission of multiple arboviruses, in particular, dengue and Zika viruses, which have recently spread through many American countries. Cross reactivity among Flaviviruse...Publisher Full TextSeveral countries have local transmission of multiple arboviruses, in particular, dengue and Zika viruses, which have recently spread through many American countries. Cross reactivity among Flaviviruses is high and present a challenge for accurate identification of the infecting agent. Thus, we evaluated the level of cross reactivity of anti-dengue IgM/G Enzyme-Linked Immunosorbent Assays (ELISA) from three manufacturers against 122 serum samples obtained at two time-points from 61 patients with non-dengue confirmed Zika virus infection. All anti-dengue ELISAs cross reacted with serum from patients with acute Zika infection at some level and a worrisome number of seroconversion for dengue IgG and IgM was observed. These findings may impact the interpretation of currently standard criteria for dengue diagnosis in endemic regions. This article is protected by copyright. All rights reserved.

  • ER protein SCAP inhibits Dengue virus NS2B3 protease by suppressing its K27-linked polyubiquitylation.
    ER protein SCAP inhibits Dengue virus NS2B3 protease by suppressing its K27-linked polyubiquitylation. [Journal Article]J Virol 2017 Feb 22.JVLiu H, Zhang L, Sun J, et al. Dengue viruses (DENVs) are an emerging threat to global public health. The NS2B3 protease complex of DENV has recently been shown to cleave antiviral protein STING and thereby subverting the innate imm...Publisher Full TextDengue viruses (DENVs) are an emerging threat to global public health. The NS2B3 protease complex of DENV has recently been shown to cleave antiviral protein STING and thereby subverting the innate immune signaling to facilitate virus replication. Whether host cells have mechanism to counteract this virus-mediated immunosuppression is unclear. We discovered that the K27-linked poly-ubiquitination of NS3 protein facilitates its recruitment of NS2B and the formation of NS2B3, and consequently the enhanced cleavage of STING. However, an ER protein SCAP, through binding to NS2B protein, inhibits the ubiquitination of NS3, rendering NS2B3 protease incapable of binding and cleavage of STING. Importantly, ectopic-expression of SCAP impaired DENV infection, whereas silencing of SCAP potentiated DENV infection. Collectively, this study uncovered a novel function of SCAP to counteract the inhibitory action of DENV NS2B3 protease on the STING signaling, suggesting that modulation of SCAP levels may have therapeutic implications.IMPORTANCE This study reports the first ubiquitylation target protein in DENV: the NS3 protein and the unique role of the K27-linked poly ubiquitylation on NS3' ability to recruit NS2B and formation of the NS2B3 protease complex. Additionally, this study identified novel functions of the ER protein SCAP: one is to compete with NS2B for binding to STING; another is to inhibit the ubiquitination of NS3. Both of these functions protect STING from being cleaved by the NS2B3 protease, and thus contribute to host antiviral response.

  • Antibody responses to Zika virus infections in flavivirus-endemic environments.
    Antibody responses to Zika virus infections in flavivirus-endemic environments. [Journal Article]Clin Vaccine Immunol 2017 Feb 22.CVKeasey SL, Pugh CL, Jensen SM, et al. Zika virus (ZIKV) infections occur in areas where dengue (DENV), West Nile (WNV), yellow fever (YFV), and other viruses of the genus Flavivirus co-circulate. The envelope protein (E) of these closely r...Publisher Full TextZika virus (ZIKV) infections occur in areas where dengue (DENV), West Nile (WNV), yellow fever (YFV), and other viruses of the genus Flavivirus co-circulate. The envelope protein (E) of these closely related flaviviruses induces specific long-term immunity, yet subsequent infections are associated with cross-reactive antibody responses that may enhance disease susceptibility and severity. To gain a better understanding of ZIKV infections against a background of similar viral diseases, we examined serological immune responses to ZIKV, WNV, DENV and YFV infections of humans and non-human primates (NHP). Using printed microarrays, we detected very specific antibody responses to primary infections with probes of recombinant E proteins from fifteen species and lineages of flaviviruses pathogenic to humans, while high cross-reactivity between ZIKV and DENV was observed with eleven printed native viruses. Notably, antibodies from human primary ZIKV or secondary DENV infections that occurred in flavivirus-endemic areas broadly recognized E proteins from many flaviviruses, especially DENV, indicating a strong influence of infection history on immune responses. A predictive algorithm was used to tentatively identify previous encounters with specific flaviviruses based on serum antibody interactions with the multi-species panel of E proteins. These results illustrate the potential impact of exposures to related viruses on the outcome of ZIKV infection, and offer considerations for development of vaccines and diagnostics.

  • Dengue infection in the nervous system: lessons learned for Zika and Chikungunya.
    Dengue infection in the nervous system: lessons learned for Zika and Chikungunya. [Journal Article]Arq Neuropsiquiatr 2017 Feb; 75(2):123-126.ANPuccioni-Sohler M, Roveroni N, Rosadas C, et al. Dengue, Zika and Chikungunya are emerging arboviruses and important causes of acute febrile disease in tropical areas. Although dengue does not represent a new condition, a geographic expansion over ti...Publisher Full TextDengue, Zika and Chikungunya are emerging arboviruses and important causes of acute febrile disease in tropical areas. Although dengue does not represent a new condition, a geographic expansion over time has occurred with the appearance of severe neurological complications. Neglect has allowed the propagation of the vector (Aedes spp), which is also responsible for the transmission of other infections such as Zika and Chikungunya throughout the world. The increased number of infected individuals has contributed to the rise of neurological manifestations including encephalitis, myelitis, meningitis, Guillain-Barré syndrome and congenital malformations such as microcephaly. In this narrative review, we characterize the impact of the geographic expansion of the vector on the appearance of neurological complications, and highlight the lack of highly accurate laboratory tests for nervous system infections. This represents a challenge for public health in the world, considering the high number of travelers and people living in endemic areas.

  • Guillain-Barré syndrome and other neurological manifestations possibly related to Zika virus infection in municipalities from Bahia, Brazil, 2015.
    Guillain-Barré syndrome and other neurological manifestations possibly related to Zika virus infection in municipalities from Bahia, Brazil, 2015. [Journal Article]Epidemiol Serv Saude 2017 Jan-Mar; 26(1):9-18.ESMalta JM, Vargas A, Leite PL, et al. most cases reported a clinical picture consistent with acute Zika virus disease, which preceded the occurrence of neurological symptoms.Publisher Full Textto describe the reported cases of Guillain-Barré Syndrome (GBS) and other neurological manifestations with a history of dengue, chikungunya or Zika virus infections, in the Metropolitan Region of Salvador and in the municipality of Feira de Santana, Brazil.this is a descriptive study with data of an investigation conducted by the epidemiological surveillance from March to August 2015; to confirm the neurological manifestations, medical diagnosis records were considered, and to prior infection, clinical and laboratory criteria were used.138 individuals were investigated, 57 reported infectious process up to 31 days before neurological symptoms - 30 possibly due to Zika, 13 to dengue, 8 to chikungunya and 6 were inconclusive -; GBS was the most frequent neurological condition (n=46), with predominance of male sex (n=32) and the median age was 44.most cases reported a clinical picture consistent with acute Zika virus disease, which preceded the occurrence of neurological symptoms.

  • Analysis of spatial mobility in subjects from a Dengue endemic urban locality in Morelos State, Mexico.
    Analysis of spatial mobility in subjects from a Dengue endemic urban locality in Morelos State, Mexico. [Journal Article]PLoS One 2017; 12(2):e0172313.PlosFalcón-Lezama JA, Santos-Luna R, Román-Pérez S, et al. Results of this study show that human mobility in a small urban setting exceeded that considered by local health authority's administrative limits, and was different between recently infected and non-i...Publisher Full TextMathematical models and field data suggest that human mobility is an important driver for Dengue virus transmission. Nonetheless little is known on this matter due the lack of instruments for precise mobility quantification and study design difficulties.We carried out a cohort-nested, case-control study with 126 individuals (42 cases, 42 intradomestic controls and 42 population controls) with the goal of describing human mobility patterns of recently Dengue virus-infected subjects, and comparing them with those of non-infected subjects living in an urban endemic locality. Mobility was quantified using a GPS-data logger registering waypoints at 60-second intervals for a minimum of 15 natural days.Although absolute displacement was highly biased towards the intradomestic and peridomestic areas, occasional displacements exceeding a 100-Km radius from the center of the studied locality were recorded for all three study groups and individual displacements were recorded traveling across six states from central Mexico. Additionally, cases had a larger number of visits out of the municipality´s administrative limits when compared to intradomestic controls (cases: 10.4 versus intradomestic controls: 2.9, p = 0.0282). We were able to identify extradomestic places within and out of the locality that were independently visited by apparently non-related infected subjects, consistent with houses, working and leisure places.Results of this study show that human mobility in a small urban setting exceeded that considered by local health authority's administrative limits, and was different between recently infected and non-infected subjects living in the same household. These observations provide important insights about the role that human mobility may have in Dengue virus transmission and persistence across endemic geographic areas that need to be taken into account when planning preventive and control measures. Finally, these results are a valuable reference when setting the parameters for future mathematical modeling studies.

  • Dengue-associated hemophagocyticlymphohistiocytosis in an adult: A case report and literature review.
    Dengue-associated hemophagocyticlymphohistiocytosis in an adult: A case report and literature review. [Journal Article]Medicine (Baltimore) 2017 Feb; 96(8):e6159.MChung SM, Song JY, Kim W, et al. It would be important to differentially diagnose dengue-associated HLH from severe DHF. Early recognition and initiation of steroid treatment would be crucial for the successful treatment of dengue fev...Publisher Full TextInfection-associated hemophagocytic syndrome (IAHS) is potentially a fatal disease caused by systemic infection complicated by hemophagocyticlymphohistiocytosis (HLH). Here, we report a case of HLH associated with dengue hemorrhagic fever (DHF) after a trip to Thailand.A 33-year-old healthy female patient presented with 3 days of fever, myalgia, and skin rash. Serotype 3 dengue virus was isolated. Clinical and laboratory findings fulfilled the criteria of HLH. After the initiation of corticosteroid therapy, the patient recovered and laboratory findings were normalized.It would be important to differentially diagnose dengue-associated HLH from severe DHF. Early recognition and initiation of steroid treatment would be crucial for the successful treatment of dengue fever complicated by HLH.

  • Coarse-Grained Molecular Modeling of Solution Structure Ensemble of Dengue Virus Non-Structural Protein 5 with Small-Angle X-Ray Scattering Intensity.
    Coarse-Grained Molecular Modeling of Solution Structure Ensemble of Dengue Virus Non-Structural Protein 5 with Small-Angle X-Ray Scattering Intensity. [Journal Article]J Phys Chem B 2017 Feb 22.JPZhu G, Saw WG, Nalaparaju A, et al. An ensemble modeling scheme incorporating coarse-grained simulations with experimental small-angle X-ray scattering (SAXS) data is applied to the dengue virus 2 (DENV2) non-structural protein 5 (NS5). ...Publisher Full TextAn ensemble modeling scheme incorporating coarse-grained simulations with experimental small-angle X-ray scattering (SAXS) data is applied to the dengue virus 2 (DENV2) non-structural protein 5 (NS5). NS5 serves a key role in viral replication through its two domains that are connected by a ten-residue polypeptide segment. A set of representative structures are generated from a simulated structure pool using SAXS data fitting by the non-negativity least squares (NNLS) or the standard Ensemble Optimization Method (EOM) based on a genetic algorithm (GA). It is found that a proper low-energy threshold of the structure pool is necessary to produce a conformational ensemble of two representative structures by both NNLS and GA that agrees well with the experimental SAXS profile. The stability of the constructed ensemble is validated also by molecular dynamics simulations with an all-atom force field. The constructed ensemble successfully revealed the domain-domain orientation and the domain contacting interface of DENV2 NS5. Using experimental data fitting and additional investigations with synthesized data, it is found that the energy restraint on the conformational pool is necessary to avoid the over-interpretation of experimental data by spurious conformational representations.

  • Modified mRNA Vaccines Protect against Zika Virus Infection.
    Modified mRNA Vaccines Protect against Zika Virus Infection. [Journal Article]Cell 2017 Feb 16.CellRichner JM, Himansu S, Dowd KA, et al. The emergence of ZIKV infection has prompted a global effort to develop safe and effective vaccines. We engineered a lipid nanoparticle (LNP) encapsulated modified mRNA vaccine encoding wild-type or va...Publisher Full TextThe emergence of ZIKV infection has prompted a global effort to develop safe and effective vaccines. We engineered a lipid nanoparticle (LNP) encapsulated modified mRNA vaccine encoding wild-type or variant ZIKV structural genes and tested immunogenicity and protection in mice. Two doses of modified mRNA LNPs encoding prM-E genes that produced virus-like particles resulted in high neutralizing antibody titers (∼1/100,000) that protected against ZIKV infection and conferred sterilizing immunity. To offset a theoretical concern of ZIKV vaccines inducing antibodies that cross-react with the related dengue virus (DENV), we designed modified prM-E RNA encoding mutations destroying the conserved fusion-loop epitope in the E protein. This variant protected against ZIKV and diminished production of antibodies enhancing DENV infection in cells or mice. A modified mRNA vaccine can prevent ZIKV disease and be adapted to reduce the risk of sensitizing individuals to subsequent exposure to DENV, should this become a clinically relevant concern.

  • Dengue virus antibody database: Systematically linking serotype-specificity with epitope mapping in dengue virus.
    Dengue virus antibody database: Systematically linking serotype-specificity with epitope mapping in dengue virus. [Journal Article]PLoS Negl Trop Dis 2017 Feb 21; 11(2):e0005395.PNChaudhury S, Gromowski GD, Ripoll DR, et al. Our results support the theory that pre-existing cross-reactive memory B cells form the basis for the secondary antibody response, resulting in a broadening of the response in terms of cross-reactivity...Publisher Full TextA majority infections caused by dengue virus (DENV) are asymptomatic, but a higher incidence of severe illness, such as dengue hemorrhagic fever, is associated with secondary infections, suggesting that pre-existing immunity plays a central role in dengue pathogenesis. Primary infections are typically associated with a largely serotype-specific antibody response, while secondary infections show a shift to a broadly cross-reactive antibody response.We hypothesized that the basis for the shift in serotype-specificity between primary and secondary infections can be found in a change in the antibody fine-specificity. To investigate the link between epitope- and serotype-specificity, we assembled the Dengue Virus Antibody Database, an online repository containing over 400 DENV-specific mAbs, each annotated with information on 1) its origin, including the immunogen, host immune history, and selection methods, 2) binding/neutralization data against all four DENV serotypes, and 3) epitope mapping at the domain or residue level to the DENV E protein. We combined epitope mapping and activity information to determine a residue-level index of epitope propensity and cross-reactivity and generated detailed composite epitope maps of primary and secondary antibody responses. We found differing patterns of epitope-specificity between primary and secondary infections, where secondary responses target a distinct subset of epitopes found in the primary response. We found that secondary infections were marked with an enhanced response to cross-reactive epitopes, such as the fusion-loop and E-dimer region, as well as increased cross-reactivity in what are typically more serotype-specific epitope regions, such as the domain I-II interface and domain III.Our results support the theory that pre-existing cross-reactive memory B cells form the basis for the secondary antibody response, resulting in a broadening of the response in terms of cross-reactivity, and a focusing of the response to a subset of epitopes, including some, such as the fusion-loop region, that are implicated in poor neutralization and antibody-dependent enhancement of infection.